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Determination of Significance：
Copper (Cu) is one of the essential trace elements of human body, and is also an important part of protein and enzyme. It can exist inside and outside the red blood cells, and its main function is to assist hematopoietic, that is, to catalyze the synthesis of hemoglobin. Copper can properly promote the development of the human bone, promote the development of the human nervous system and the brain, and maintain the normal growth and development of infants and young children, therefore, the determination of copper ion content in the tissue can know whether the body is deficient in copper.
Measurement Principle：
Under acidic conditions, Cu2+ is dissociated from ceruloplasmin and albumin and reacts with complexing agent 3, 5-dibromo-PAESA to produce a purple complex, which has a characteristic absorption peak at 580nm, and the absorbance is proportional to the concentration in a certain range, thus calculating the Cu2+ concentration.
Self Provided：
Spectrophotometer/microplate reader, cryogenic centrifuge, water bath/constant temperature incubator, adjustable pipette, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, ice and distilled water
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