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CAS:1320-06-5
Storage:Powder:2-8℃，2 years
Appearance:Brown to black Solid
Oil Red O (Oil Red O) is a fat-soluble azo dye, a strong lipid solvent and stain agent, which can specifically stain neutral triglycerides, lipids and lipoproteins in tissues and cells. When the tissue section is placed in the dye solution, the dye leaves the dye solution and dissolves in the lipids (such as lipid droplets) in the tissue, making the lipid droplets in the tissue red. For the analysis of lipid status in cell samples. Operation Instructions (for reference only) Oil Red O reserve liquid Saturated isopropyl alcohol oil Red O stain solution
Oil Red O working Oil red O reserve liquid at 3:2(oil red O: distilled water) add distilled water, mix well, leave at room temperature for 5-10min, filter and use.
Cultured cell
1. The cell medium was removed, washed twice with PBS, and fixed with 10% formalin for 20-30min.
2. Discard the fixing solution, wash with distilled water twice, add 60% isopropyl alcohol for 20-30s.
3. After discarding 60% isopropyl alcohol, add the freshly prepared oil red O working liquid and soak it for 10-20min.
4. Discard the dye and rinse with 60% isopropyl alcohol for 20-30s until the interstitium is clear. Wash 2-5 times until there is no excess dye.
5. (Optional) Add hematoxylin staining solution and redye the nucleus for 1-2min. After discarding the dye, wash 2-5 times, add PBS to incubate for 1min, and discard.
6. Add distilled water to cover the cells and observe under a microscope.
Smear of cells
1. Prepare fresh smear, fix with 10% formalin for 10-15min, remove the smear and dry in the air for 10-15min.
2. Add the freshly prepared oil red O working liquid and soak it for 15min. Rinse with 60% isopropyl alcohol for 20-30s, rinse with running water, and wash slightly with distilled water.
3. (Optional) Add hematoxylin staining solution, re-stain the nucleus for 2min, add PBS to incubate for 1min, and observe the staining results under the microscope.
Matters needing attention
1. Oil red O working liquid is unstable, easy to precipitate, should not be prepared in advance, it is recommended to use now.
2. Dyeing results can not be stored for a long time, should be observed and photographed as soon as possible.
