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My Cart> Biochemical Assay Kits > REDOX Series > Antioxidant Series > UDP-Flavonoid glycosyltransferase (UFGT) Activity Assay kitDetection Method：Spectrophotometer/Microplate Reader
Storage：Store at -20℃，6 months
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| Name | UDP-Flavonoid glycosyltransferase (UFGT) Activity Assay kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
UDP-glycose flavonoid glycosyltransferase (UFGT) is the last acting enzyme of the shikimate pathway and the first acting enzyme that causes the formation of stable anthocyanins and their conversion from colorless to colored; UFGT is the key enzyme in the process of fruit coloring, which converts unstable anthocyanins to stable anthocyanin.
Measurement Principle：
UFGT catalyzes the formation of UDP and quercetin glycosides from UDPG and quercetin; UDP oxidizes NADH to NAD+ under the action of pyruvate kinase and lactate dehydrogenase, and the rate of NAD+ generation is directly proportional to the content of UDP, which is reflected by the rate of decrease of absorbance at 340 nm.
Self Provided：
Ultraviolet spectrophotometer/ microplate reader, cryogenic centrifuge, water bath/incubator, analytical balance, adjustable pipette, micro quartz cuvette/96-well UV plate, mortar/homogenizer, distilled water and ice.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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