Determination of Significance:
Histamine is a potentially harmful nitrogen containing low molecular weight organic compound, which is produced by histidine under the action of decarboxylase. Histamine is released when tissue is damaged or when inflammation and allergic reactions occur. Histamine is widely found in various foods, and excessive intake of histamine will cause harm to human body.
Measurement Principle:
Histamine is specifically broken down by histamine dehydrogenase (HDH), and under 1-mPMS, electron transfer occurs through WST for color rendering, with a maximum absorption peak at 470nm, according to which histamine content can be calculated
Self Provided:
Spectrophotometer, mortar/homogenizer/cell ultrasonic crusher, centrifuge, constant temperature foster box/water-bath, 1 mL glass cuvette, ice and distilled water.
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