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Determination of Significance：
Soil acid xylanase (S-ACX), also known as soil acidic hemicellulase, is mainly isolated from microorganisms with an optimal growth pH of 4-5.
Measurement Principle：
In acidic environments, S-ACX catalyzes the degradation of xylan into reducing oligosaccharides and monosaccharides. Under boiling water bath conditions, it further undergoes a color reaction with 3,5-dinitrosalicylic acid, with a characteristic absorption peak at 540nm. The color depth of the reaction solution is directly proportional to the amount of reducing sugars produced by enzymatic hydrolysis. By measuring the rate of increase in absorbance of the reaction solution at 540nm, S-ACX activity can be calculated.
Self Provided：
Spectrophotometer/Microplate reader, micro glass cuvette/96 well plate, balance, desk centrifuge, water bath, 30-50 mesh sieve, distilled water.
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