Berberine

Examples of using this product（for reference only）

HPLC

References：

Li H, Lv T, Wang B, Li M, Liu J, Wang C, Tang Z. Integrating Network Pharmacology and Experimental Models to Investigate the Mechanism of Huanglian Jiedu Decoction on Inflammatory Injury Induced by Cerebral Ischemia. Evid Based Complement Alternat Med. 2021 Jan 13;2021:2135394. doi: 10.1155/2021/2135394. PMID: 33519941; PMCID: PMC7817265.

UPLC-PDA

The levels of the main active ingredients of MSJZT were detected using a UPLC-PDA method. Chromatographic separation was performed on a UPLC system with a photodiode array detector (PDA) and C18 (100 mm×2.1 mm, 1.7μm ) column. The column temperature was set at 30℃. The mobile phase was composed of 0.1% phosphoric acid (A) and acetonitrile (B). The optimum adsorption and elution conditions were set as follows: 0–1.5 min, 95% B; 1.5–6 min, 95–80% B; 7–10 min, 80–60% B; 10–10.5 min, 50–35% B; 10.5–12 min, 35–30% B; 12–12.5 min, 30–20% B; 12.5–16 min, 20–10% B; 16–16.5 min, 10–0% B; and 16.5–18 min, 0–95% B.

References：

Nie S, Zhao Y, Qiu X, Wang W, Yao Y, Yi M, Wang D. Metabolomic Study on Nude Mice Models of Gastric Cancer Treated with Modified Si Jun Zi Tang via HILIC UHPLC-Q-TOF/MS Analysis. Evid Based Complement Alternat Med. 2019 Jun 23;2019:3817879. doi: 10.1155/2019/3817879. PMID: 31341492; PMCID: PMC6612382.

HPLC

HPLC was performed using an equipped with a DAD detector and a C18 (250 mm × 4.6 mm; 5 μm). The mobile phase included 0.2% phosphoric acid plus 0.4% sodium 1-heptanesulfonate (A) and methanol (B) at a flow speed of 1.0 ml/min in the condition of a column temperature of 30℃. The detection wavelength was set at 214 nm. The reference standard sample injection volume was 10 μl. The gradient elution was as follows: 0–5 min (100–100% A, 0%–0% B), 5–60 min (100–60% A, 0%–40% B), 60–120 min (60–30% A, 40–70% B), 120–125 min (30–30% A, 70–70% B), 125–125.1 min (30–100% A, 70–0% B), 125.1–132 min (100–100% A, 0–0% B). The chromatographic data and peak area scores were collected and analyzed using ChemStation software.

References：

Duan Q, Liu T, Huang C, Shao Q, Ma Y, Wang W, Liu T, Sun J, Fang J, Huang G, Chen Z. The Chinese Herbal Prescription JieZe-1 Inhibits Membrane Fusion and the Toll-like Receptor Signaling Pathway in a Genital Herpes Mouse Model. Front Pharmacol. 2021 Sep 24;12:707695. doi: 10.3389/fphar.2021.707695. PMID: 34630083; PMCID: PMC8497740.

HPLC

The HPLC system was equipped with a quaternary pump and UV-Vis detector . The chromatographic separation was carried out on an C18 column (250 × 4.6 mm i.d., 5 μm). Mobile phase consisted of 50% solution (v/v) of acetonitrile in water (0.1% H3PO4 and 0.1% SDS). The flow rate was 1 mL/min and the column temperature was set to 40℃. The effluent was monitored at 265 nm and the injection volume was 20 μL.

References：

Yang L, Tian J, Meng J, Zhao R, Li C, Ma J, Jin T. Modification and Characterization of Fe?O? Nanoparticles for Use in Adsorption of Alkaloids. Molecules. 2018 Mar 2;23(3):562. doi: 10.3390/molecules23030562. PMID: 29498692; PMCID: PMC6017166.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.