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My CartReactivity：Human,Mouse,Rat
Application：IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：3418
Host：Rabbit
Qty：
Size：
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Aspergillus niger;Cow;Monkey |
| Dilution Ratio | WB 1:1000-3000. IHC 1:50-200. |
| Target | IDH2 |
| Background | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants. |
| Swiss Prot | P48735 |
| Gene ID | 3418 |
| Subcellular Location | Cytoplasm |
| Immunogen | A synthetic peptide of human IDH2 |
| Public Immunogen Range | 100-150/452aa |
| Calculated MW | 45/51kDa |
| Observed MW | 51kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Mouse Rat |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with IDH2 antibody diluted at 1:2000;Lane: HeLa,MCF7,NIH3T3,SH-SY5Y,U-87,Mouse kidney,Mouse heart,Mouse brain,Mouse liver,Rat liver,Rat lung,Rat kidney,Mouse skeletal muscle.
Immunohistochemistry of paraffin-embedded Human breast cancer with IDH2 antibody diluted at 1:100.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with IDH2 antibody diluted at 1:100.
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