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My CartReactivity：Human,Rat,Mouse
Application：IF,FCM,WB,IHC,ELISA
Clone Type：Polyclonal Antibody
Appearance：液体
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：5465
Host：Rabbit
Qty：
Size：
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-2000. IHC 1:100-500. IF 1:100-500. FCM 1μg /test. ELISA 1:5000-10000. |
| Target | PPAR alpha |
| Background | Peroxisome proliferators are nongenotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed Peroxisome Proliferator Activated Receptors (PPARs). Nuclear hormone receptors are ligand dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids. Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis. PPAR alpha, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex. |
| Swiss Prot | Q07869 |
| Gene ID | 5465 |
| Subcellular Location | Nucleus |
| Immunogen | A synthetic peptide of human PPAR alpha |
| Public Immunogen Range | 301-400/468aa |
| Calculated MW | 51kDa |
| Purification Method | Affinity purification |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA，0.03% Proclin300 and 50% Glycerol. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Appearance | 液体 |
| Unit | Piece |
| Y-Dilution Ratio | WB 1:500-2000. IHC 1:100-500. IF 1:100-500. FCM 1米g /test.?ELISA 1:5000-10000. |
| Specification | 50ul 100ul |
| Reactivity | Human Mouse Rat |
| Application | FCM IF IHC ELISA WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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