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My CartReactivity：Human,Mouse,Rat
Application：IHC,WB
Clone Type：Polyclonal Antibody
Appearance：液体
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：6232
Host：Rabbit
Qty：
Size：
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-2000. IHC 1:50-200. |
| Target | RPS27 |
| Background | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the S27e family of ribosomal proteins and component of the 40S subunit. The encoded protein contains a C4-type zinc finger domain that can bind to zinc and may bind to nucleic acid. Studies in rat indicate that ribosomal protein S27 is located near ribosomal protein S18 in the 40S subunit and is covalently linked to translation initiation factor eIF3. Mutations in this gene have been identified in numerous melanoma patients and in at least one patient with Diamond-Blackfan anemia (DBA). Elevated expression of this gene has been observed in various human cancers. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. |
| Swiss Prot | P42677 |
| Gene ID | 6232 |
| Subcellular Location | Cytosol Nucleus |
| Immunogen | Recombinant protein of human RPS27 |
| Calculated MW | 9kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Appearance | 液体 |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human Mouse Rat |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with RPS27 antibody diluted at 1:1000;Lane: Mouse heart,HepG2,293T,HeLa,A549.
Immunohistochemistry of paraffin-embedded Human prostate cancer with RPS27 antibody diluted at 1:100.
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