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My CartReactivity：Human,Mouse,Monkey
Application：IF,IHC,WB
Clone Type：Polyclonal Antibody
Appearance：液体
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：4191
Host：Rabbit
Qty：
Size：
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Arabidopsis thaliana |
| Dilution Ratio | WB 1:1000-3000. IHC 1:50-200. IF 1:50-200. |
| Target | MDH2 |
| Background | Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Several transcript variants encoding different isoforms have been found for this gene. |
| Swiss Prot | P40926 |
| Gene ID | 4191 |
| Subcellular Location | Cytoplasm Nucleus Secreted |
| Immunogen | A synthetic peptide of human MDH2 |
| Public Immunogen Range | 80-130/338aa |
| Calculated MW | 31/36kDa |
| Observed MW | 33/34kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Appearance | 液体 |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Monkey Mouse |
| Application | WB IHC IF |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with MDH2 antibody diluted at 1:2000;Lane: 293T,HeLa,HepG2,U-87MG,C2C12,NIH3T3,A549,COS7,K-562,Mouse brain,Mouse heart,Mouse lung.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with MDH2 antibody diluted at 1:100.
Immunofluorescence analysis of NIH3T3 cells using MDH2 antibody diluted at 1:100.
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