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My CartReactivity：Human,Rat,Mouse
Application：IHC,WB
Clone Type：Polyclonal Antibody
Appearance：液体
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：4719
Host：Rabbit
Qty：
Size：
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Bovine;Monkey;Gorilla;Chimpanzee;Orangutan |
| Dilution Ratio | WB 1:1000-3000. IHC 1:50-200. |
| Target | NDUS1 |
| Background | The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. Several transcript variants encoding different isoforms have been found for this gene. |
| Swiss Prot | P28331 |
| Gene ID | 4719 |
| Subcellular Location | Cytoplasm |
| Immunogen | Recombinant protein of human NDUS1 |
| Public Immunogen Range | 1-256aa |
| Calculated MW | 68/74/75/79/81kDa |
| Observed MW | 81kDa |
| Purification Method | Octanoic acid-ammonium sulfate precipitation |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Appearance | 液体 |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Mouse Rat Human |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with NDUS1 antibody diluted at 1:2000;Lane: HeLa,Raji,PC-3M-IE8.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with NDUS1 antibody diluted at 1:100.
Immunohistochemistry of paraffin-embedded Human liver cancer with NDUS1 antibody diluted at 1:100.
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