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My CartReactivity：Mouse,Rat,Human
Application：WB
Clone Type：Polyclonal Antibody
Appearance：液体
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：5562/5563
Host：Rabbit
Qty：
Size：
| Name | Anti-PRKAA1/PRKAA2 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Pig;Chimpanzee |
| Dilution Ratio | WB 1:1000-3000. |
| Target | PRKAA1/PRKAA2 |
| Background | The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. |
| Swiss Prot | Q13131/P54646 |
| Gene ID | 5562/5563 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | A synthetic peptide of human PRKAA1/PRKAA2 |
| Public Immunogen Range | 150-200/559aa |
| Calculated MW | 62/64/66kDa |
| Observed MW | 65kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Appearance | 液体 |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Mouse Rat Human |
| Application | WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with PRKAA1/PRKAA2 antibody diluted at 1:2000;Lane: HeLa,MCF7,A549,293T,NIH3T3.
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