English
中文
CAS:491-70-3
Appearance:Light yellow to yellow powder
Storage:RT,avoid light,2-8℃
Product Introduction
This product is an antimicrobial (microbicide) and preservative (preservative) with broad spectrum, high efficiency, stability and low toxicity. It can be used as a preservative for primary and secondary antibodies, sealing liquid, antibody diluent, washing liquid, biochemical buffer, water for water bath, cleaning agent, etc. The main active ingredient is 2-methyl-4-isothiazloin-3-one (2-methyl-4-isothiazloin-3-one, MIT). Two ingredients, MIT and 5-chloro-2-methyl-4-isothiazolin-3-one (5-Chloro-2-methyl-4-isothiazolin-3-one (CMIT), inhibit microbial growth and promote microbial death. The idea is that when CMIT/MIT comes into contact with the cell membrane, It can immediately penetrate into the cell membrane and inhibit α-ketoglutarate dehydrogenase and succinate dehydrogenase during the tricarboxylic acid cycle, the key stages of cell metabolism dehydrogenase) and NADH dehydrogenase activity, meanwhile, Other important enzymes such as pyruvate dehydrogenase, lactate dehydrogenase, and alcohol dehydrogenase can also be inhibited, Thus inhibiting cell metabolism and the synthesis of macromolecules, resulting in a rapid decline in intracellular energy levels, and eventually growth inhibition or death due to the collapse of the energy system and the inability of cells to carry out normal anabolism. Bacteria and fungi have all or part of the tricarboxylic acid cycle, so this product is suitable for both bacteria and fungi, and is a perfect alternative to traditional biological preservatives such as thiomersal, sodium azide and gentamicin.
Product Features
1. This product is a broad-spectrum antibacterial preservative with fast onset time, long duration of efficacy and rarely resistance.
2. This product is suitable for a wide range of pH, pH≤3.5 or > 10.5 can also inhibit bacteria, compatible with various enzymes, does not inhibit antibody binding.
3. This product has high stability and is more stable at high temperature. It is compatible with most raw materials or formulations, such as anionic, cationic, non-ionic surfactants, etc. It is completely insoluble with water and suitable for aqueous solution formulation without interference to the color of reagents.
4. This product has low toxicity, does not contain formaldehyde, has good biodegradation ability, will not cause pollution to the environment.
Instructions for Use
The applicable pH range of this product is 2-12, and the recommended concentration is 0.05-0.10% (or about 0.5~1ml/L), and the addition amount can be increased to 0.1-0.2% for some solutions that are particularly easy to be contaminated by microorganisms. The dosage depends on the microbial species, pH, temperature and storage life, and it is recommended to determine the best dose by experiment.
