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CAS:480-18-2
Appearance: White to off-white Powder
Storage:RT,avoid light,2-8℃
Product Introduction
This product is an antimicrobial (microbicide) and preservative (preservative) with broad spectrum, high efficiency, stability and low toxicity. It can be used as a preservative for primary and secondary antibodies, sealing liquid, antibody diluent, washing liquid, biochemical buffer, water for water bath, cleaning agent, etc. The main active ingredients are 5-chloro-2-methyl-4-isothiazolin-3-one (5-chloro-2-methyl-4-isothiazolin-3-one, CMIT) and 2-methyl-4-isothiazolin-3-one (2-methyl-4-isothiazloin-3-one, CMIT). MIT's 3:1 mixture. These two components can inhibit microbial growth and promote microbial death. The idea is that when CMIT/MIT comes into contact with the cell membrane, It can immediately penetrate into the cell membrane and inhibit α-ketoglutarate dehydrogenase and succinate dehydrogenase during the tricarboxylic acid cycle, the key stages of cell metabolism dehydrogenase) and NADHdehydrogenase activity, meanwhile, Other important enzymes such as pyruvate dehydrogenase, lactatedehydrogenase, and alcohol dehydrogenase can also be inhibited, inhibiting cell metabolism and macromolecule synthesis. It causes a rapid decline in the energy level of the cell, and eventually growth inhibition or death due to the breakdown of the energy system and the inability of the cell to carry out normal anabolism. Bacteria and fungi have all or part of the tricarboxylic acid cycle, so this product is suitable for both bacteria and fungi, and is a perfect alternative to traditional biological preservatives such as thiomersal, sodium azide and gentamicin.
Product Features
1. This product is a broad-spectrum antibacterial preservative with fast onset time, long duration of efficacy and rarely resistance.
2. This product is suitable for a wide range of pH, good compatibility with antibodies and some enzymes, and can perfectly replace traditional biological preservatives such as thiomersal, sodium azide and gentamicin.
3. This product has high stability, compatible with most raw materials or formulations, such as anionic, cationic, non-ionic surfactants, etc.
There is no interference with the color of the reagent.
4. This product has low toxicity, does not contain formaldehyde, has good biodegradation ability, will not cause pollution to the environment.
Instructions for Use
The applicable pH range of this product is 2.5-8.5, the recommended concentration is 0.05-0.10% (or about 0.5~1ml/L), and the addition amount can be increased to 0.1-0.2% for some solutions that are particularly easy to be contaminated by microorganisms. The dosage depends on the microbial species, pH, temperature and storage life, and it is recommended to determine the best dose by experiment.
