Reduced Serum RPMI 1640 Medium

Product Introduction

Attenuated serum RPMI 1640 medium is commonly used in attenuated mammalian cell cultures and contains phenol red and is free of L-glutamine and HEPES. Compared with classical DMEM, this formula can reduce the amount of serum by more than 50%, and does not affect the cell growth rate and morphology, thus extending the time of use of the same batch of serum, and reduce the fluctuation of culture conditions, but also reduce the infection and transmission of pathogens, thereby improving the purity and reliability of cell culture. The medium can be used for HeLa cells, Jurkat cells, MCF-7 cells, PC12 cells, PBMC cells, astrocytes and cancer cells without domestication. Compared to other media, RPMI 1640 medium contains the reducing agents glutathione and biotin and a high concentration of vitamins such as vitamin B12 and PABA.

Instructions for Use

RPMI1640 medium is free of proteins, lipids and growth factors. Therefore, RPMI1640 medium usually requires the addition of 1% to 5% fetal bovine serum (FBS) when used.

Precautions

This product is filtered to remove bacteria, and should pay attention to aseptic operation when using to avoid contamination; In order to maintain the best use of this product, do not freeze and thaw treatment; This product is only for scientific research and production use, for in vitro culture of tissues and cells, clinical use is prohibited; Cells need to be grown in an incubator containing 5 to 10 percent carbon dioxide.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.