In botanical experiments, different hierarchical structures of plant tissues cannot be observed by traditional methods, because the absorption of light waves by pigments in the sample and the different refractive indices of light by various parts of the sample cause light scattering, which is not conducive to observation. In order to better observe the internal structure of plant tissues, a large number of tissue transparency techniques have been developed and applied in the field of plant tissue imaging in the past two decades to observe bacteria and fluorescent proteins inside and outside tissues. At present, the commonly used plant tissue transparency methods can be divided into organic solvent based and water-based solvent based transparency methods, of which chloral hydrate is the most commonly used method, but because of its special smell, it is not friendly to users.

The transparent liquid of plant tissue (rapid) adopts the transparent method based on organic solvents, has high lipid solubility and high refractive index (RI=1.5), can effectively replace chloral hydrate, and is suitable for the overall transparency of thin and light young leaf tissues, and the transparent time is short, generally within 1-3 days to achieve complete transparency; The structure is relatively intact. The reagent is intended for scientific research only and is not intended for clinical diagnosis or other purposes.