CAS:438056-69-0,9001-99-4
Appearance:White to nearly white to light yellow powder or crystal,≥ 90%
Storage:piece
Purity:97.00%,14.3kDa
Introduction
RibonucleaseA (RNase A) is A non-specific endonuclease derived from bovine pancreas modified by protein engineering technology and obtained by yeast expression and purification. Functionally, RNase A is an endonuclease that hydrolyzes the phosphodiester bond between the 5 '-ribose on the nucleoside and the phosphate group on the adjacent 3' -ribose pyrimidine nucleoside, resulting in the hydrolysis of the 2 ',3 '-cyclic phosphoric acid to the corresponding 3' -nucleoside phosphate. Therefore, single-stranded RNA can be specifically degraded at the sites of C and U nucleotide residues. RNase A is very stable. RNase A is most efficient when it acts on single-stranded RNA.
Application
Remove RNA contamination from DNA or protein samples.
How to use
Storage concentration:10 mg/mL.
The working concentration:10-20μg/mL, the working liquid should be diluted with diluent or the working substrate should be 20 mM Mg2+.