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Storage:Store at 2-8℃，1 year
Appearance:Yellow transparent solution
CAS:9001-99-4
Introduction
RibonucleaseA (RNase A) is A non-specific endonuclease derived from bovine pancreas modified by protein engineering technology and obtained by yeast expression and purification. It does not contain bacterial endotoxins expressed by prokaryotes. Functionally, RNase A is an endonuclease that hydrolyzes the phosphodiester bond between the 5 '-ribose on the nucleoside and the phosphate group on the adjacent 3' -ribose pyrimidine nucleoside, resulting in the hydrolysis of the 2 ',3 '-cyclic phosphoric acid to the corresponding 3' -nucleoside phosphate. Therefore, single-stranded RNA can be specifically degraded at the sites of C and U nucleotide residues. RNase A is very stable. RNase A is most efficient when it acts on single-stranded RNA.
Application
Remove RNA contamination from DNA or protein samples
How to use
Storage concentration 10 mg/mL. The working concentration is 10-20 μg /mL, the working liquid should be diluted with diluent or the working substrate should be 20 mM Mg2+.
