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Your Position: Home > Protein and Immunity > Recombinant Protein > 重组核糖核酸酶A（Recombinant RNaseA）
重组核糖核酸酶A（Recombinant RNaseA）

CAS:898543-06-1,9001-99-4

Appearance:White to grayish white crystalline powder,bottle

Storage:14.3kDa

Purity:97.00%

重组核糖核酸酶A（Recombinant RNaseA）
Cat.No：
R8023
Brand：
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
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Product Information Related Products Related Paper COA Technical Q&A

Introduction

RibonucleaseA (RNase A) is A non-specific endonuclease derived from bovine pancreas modified by protein engineering technology and obtained by yeast expression and purification. It does not contain bacterial endotoxins expressed by prokaryotes. Functionally, RNase A is an endonuclease that hydrolyzes the phosphodiester bond between the 5 '-ribose on the nucleoside and the phosphate group on the adjacent 3' -ribose pyrimidine nucleoside, resulting in the hydrolysis of the 2 ',3 '-cyclic phosphoric acid to the corresponding 3' -nucleoside phosphate. Therefore, single-stranded RNA can be specifically degraded at the sites of C and U nucleotide residues. RNase A is very stable. RNase A is most efficient when it acts on single-stranded RNA.

Application

Remove RNA contamination from DNA or protein samples

How to use

Storage concentration 10 mg/mL. The working concentration is 10-20 μg /mL, the working liquid should be diluted with diluent or the working substrate should be 20 mM Mg2+.

Remark：
These protocols are for reference only. Solarbio does not independently validate these methods.

Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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