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My CartReactivity：Human,Rat,Mouse
Application：WB,IHC,IF,IP,FCM,ELISA
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：7082
Host：Rabbit
Qty：
Size：
| Name | Anti-TJP1 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:5000-50000. IHC 1:1000-4000. IF 1:1000-4000. IP 0.5-4.0 ug. FCM 0.20 ug per 10^6 cells in a 100 μl suspension. |
| Target | TJP1 |
| Background | This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017] |
| Swiss Prot | Q07157 |
| Gene ID | 7082 |
| Subcellular Location | Cytoplasm Cell membrane |
| Immunogen | Recombinant protein of human ZO-1 |
| Public Immunogen Range | 1446-1748aa |
| Calculated MW | 195kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Y-Dilution Ratio | WB 1:5000-50000. IHC 1:1000-4000. IF 1:1000-4000. IP 0.5-4.0ug. FCM 1X10^6cells+0.20 ug. |
| Specification | 50ul 100ul |
| Reactivity | Human Rat Mouse |
| Application | WB ELISA FCM IP IF IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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