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My CartReactivity：Human,Rat,Mouse
Application：WB,IF,IHC
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：4803
Host：Rabbit
Qty：
Size：
| Name | Anti-NGF Monoclonal Antibody |
| Host | Rabbit |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-1000. IHC 1:100-200. IF 1:50-200. |
| Target | NGF |
| Background | Nerve growth factor (NGF) is a small, secreted protein and member of the neurotrophin family of growth factors that promote neuronal cell survival and differentiation. Producing cells release NGF that bind and activate TrkA high affinity receptors to mediate NGF-driven signaling. NGF also binds to a low affinity p75 (NTR) receptors, which belong to the death receptor family. |
| Swiss Prot | P01138 |
| Gene ID | 4803 |
| Subcellular Location | Secreted |
| Immunogen | A synthetic peptide of human NGF |
| Calculated MW | 27kDa |
| Purification Method | Affinity purification |
| Storage Buffer | Liquid in PBS，pH 7.4，150mM NaCl，0.02% sodium azide and 50% glycerol. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human Mouse Rat |
| Application | WB IHC IF |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with NGF antibody diluted at 1:1000;Lane: Mouse thyroid,HeLa.
Immunohistochemistry of paraffin-embedded Human kidney with NGF antibody diluted at 1:150.
Immunofluorescence analysis of SH-SY5Y cells using NGF antibody diluted at 1:100.
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