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My CartCAS：73-22-3
Purity：HPLC≥98%
Molecular Formula：C11H12N2O2
Molecular Weight：204.23
Appearance：White to off-white powder
Storage：Store at RT，2 years.
Qty：
Size：
| CAS | 73-22-3 |
| Name | L-Tryptophan |
| Purity | HPLC≥98% |
| Molecular Formula | C11H12N2O2 |
| Molecular Weight | 204.23 |
| Appearance | White to off-white powder |
| Solubility | ≥10mg/mL in Water |
| Storage | Store at RT，2 years. |
| EC | EINECS 200-795-6 |
| MDL | MFCD00064340 |
| SMILES | N[C@@H](CC1=CNC2=CC=CC=C12)C(O)=O |
| InChI | InChI=1S/C11H12N2O2/c12-9(11(14)15)5-7-6-13-10-4-2-1-3-8(7)10/h1-4，6，9，13H，5，12H2，(H，14，15)/t9-/m0/s1 |
| Note | The reserve liquid is recommended to be packaged and frozen to avoid repeated freezing and thawing (-20℃, 1 month); The diluted working liquid is recommended to be used and prepared immediately |
| Unit | Bottle |
| Specification | 200mg |
Examples of using this product（for reference only）
HPLC-MS
HPLC was performed using an HPLC-MS instrumen for detection and quantitation of tryptophan and kynurenine. The mass scan mode was set to positive and negative ions, and the range was set to 70–1000 m/z. Separation of tryptophan and kynurenine was carried out on an C18 column. Standards and samples were eluted using methyl alcohol and ultrapure water (0.1% formic acid). The injection volume was set to 5 μL, with a solvent flow rate of 0.2 mL/min. The column temperature was set to 40 ℃. The IDA mode was used for data acquisition.
References：
Ai Y, Wang B, Xiao S, Luo S, Wang Y. Tryptophan Side-Chain Oxidase Enzyme Suppresses Hepatocellular Carcinoma Growth through Degradation of Tryptophan. Int J Mol Sci. 2021 Nov 18;22(22):12428. doi: 10.3390/ijms222212428. PMID: 34830310; PMCID: PMC8623686.
HPLC
The SA content was determined using high-performance liquid chromatography with a 4.6 × 250 mm column . The mobile phase had a flow rate of 0.7 mL/min (isocratic elution) and was composed of acetonitrile and 0.1% phosphoric acid (97:3, v/v). The column temperature was 25 ℃, a UV–visible detector was used for detection at a wavelength of 213 nm, and the injection volume was 20 μL.
References：
Shi Y, Chen G, Chen K, Chen X, Hong Q, Kan J. Assessment of fresh star anise (Illicium verum Hook.f.) drying methods for influencing drying characteristics, color, flavor, volatile oil and shikimic acid. Food Chem. 2021 Apr 16;342:128359. doi: 10.1016/j.foodchem.2020.128359. Epub 2020 Oct 13. PMID: 33092919.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Publish_to：PHYTOMEDICINE
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Metabolites analysis of plantamajoside based on gut microbiota-drug interaction
Click to check >>Author：Hui Xu, Hang Yu, Jie Fu, Zheng-Wei Zhang, Jia-Chun Hu, Jin-Yue Lu, Xin-Yu Yang, Meng-Meng Bu, Jian-Dong Jiang, Yan Wang
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Publish_to：PHYTOMEDICINE
PMID：37196513
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Click to check >>Author：Nan Jiang, Shujuan Hou, Yuye Liu, Peixing Ren, Nuoyu Xie, Ye Yuan, Qing Hao, Mengjun Liu, Zhihui Zhao
IF：6.6270
Publish_to：Frontiers in Plant Science
PMID：37265633
Tryptophan Side-Chain Oxidase Enzyme Suppresses Hepatocellular Carcinoma Growth through Degradation of Tryptophan
Click to check >>Author：Ai Y; Wang B; Xiao S; Luo S; Wang Y.
IF：6.2080
Publish_to：Int J Mol Sci
PMID：34830310
Manual Download
氨基酸类原料，能否用于细胞培养 2023-09-19 10:48:06
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