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My CartReactivity：Human,Mouse,Rat
Application：IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：4190
Host：Rabbit
Qty：
Size：
| Name | Anti-MDH1 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Pig |
| Dilution Ratio | WB 1:8000-12000. IHC 1:50-200. |
| Target | MDH1 |
| Background | This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. Alternatively spliced transcript variants have been found for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 6. |
| Swiss Prot | P40925 |
| Gene ID | 4190 |
| Subcellular Location | Cytoplasm |
| Immunogen | Recombinant protein of human MDH1 |
| Public Immunogen Range | 1-335aa |
| Calculated MW | 27/36/39kDa |
| Observed MW | 37kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Mouse Rat |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with MDH1 antibody diluted at 1:10000;Lane: HepG2,U937,PC3,Jurkat,NIH3T3,Raw264.7,Mouse liver,Mouse brain,Mouse lung,Mouse heart,Mouse kidney,Mouse eye,Rat kidney,Rat liver,Rat heart,Rat brain.
Immunohistochemistry of paraffin-embedded Human liver cancer with MDH1 antibody diluted at 1:100.
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