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My CartReactivity：Mouse,Rat,Human
Application：WB,IHC
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：7137
Host：Rabbit
Qty：
Size：
| Name | Anti-TNNI3 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Bovine;Rabbit;Horse |
| Dilution Ratio | WB 1:1000-3000. IHC 1:50-200. |
| Target | TNNI3 |
| Background | Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). |
| Swiss Prot | P19429 |
| Gene ID | 7137 |
| Subcellular Location | Cytoplasm |
| Immunogen | Recombinant protein of human TNNI3 |
| Public Immunogen Range | 1-211aa |
| Calculated MW | 24kDa |
| Observed MW | 26kDa |
| Purification Method | Octanoic acid-ammonium sulfate precipitation |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Mouse Rat |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with TNNI3 antibody diluted at 1:2000;Lane: Mouse heart,Rat heart.
Immunohistochemistry of paraffin-embedded Mouse heart tissue with TNNI3 antibody diluted at 1:100.
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