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My CartReactivity：Rat,Monkey,Human,Mouse
Application：IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：9101
Host：Rabbit
Qty：
Size：
| Name | Anti-USP8 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Bovine;Horse;Rabbit |
| Dilution Ratio | WB 1:2000-5000. IHC 1:50-200. |
| Target | USP8 |
| Background | The encoded protein is thought to regulate the morphology of the endosome by ubiquitination of proteins on this organelle and is involved in cargo sorting and membrane trafficking at the early endosome stage. This protein is required for the cell to enter the S phase of the cell cycle and also functions as a positive regulator in the Hedgehog signaling pathway in development. Pseudogenes of this gene are present on chromosomes 2 and 6. Alternate splicing results in multiple transcript variants. |
| Swiss Prot | P40818 |
| Gene ID | 9101 |
| Subcellular Location | Cytoplasm Nucleus Cell membrane |
| Immunogen | Recombinant protein of human USP8 |
| Public Immunogen Range | 375-724aa |
| Calculated MW | 115/128kDa |
| Observed MW | 128-135kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Monkey Rat Mouse |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with USP8 antibody diluted at 1:3000;Lane: U937,HEL,HCT116,MCF7,A549,COS7,HeLa,HepG2,NIH3T3,Jurkat,U-251MG,Mouse testis,Rat testis.
Immunohistochemistry of paraffin-embedded Human pancreatic cancer with USP8 antibody diluted at 1:100.
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