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My CartReactivity：Human,Mouse,Rat
Application：IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：89869
Host：Rabbit
Qty：
Size：
| Name | Anti-PLCZ1 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Chicken;Bovine;Monkey |
| Dilution Ratio | WB 1:1000-3000. IHC 1:50-200. |
| Target | PLCZ1 |
| Background | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca2+-dependent manner. Triggers intracellular Ca2+ oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function. |
| Swiss Prot | Q86YW0 |
| Gene ID | 89869 |
| Subcellular Location | Nucleus Cytoplasm |
| Immunogen | Recombinant protein of human PLCZ1 |
| Public Immunogen Range | 60-340aa |
| Calculated MW | 48/58/70kDa |
| Observed MW | 65kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Mouse Rat |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with PLCZ1 antibody diluted at 1:2000;Lane: PC-3M-IE8,HEL,U-251MG.
Immunohistochemistry of paraffin-embedded Human endometrial cancer with PLCZ1 antibody diluted at 1:100.
Immunohistochemistry of paraffin-embedded Human lung cancer with PLCZ1 antibody diluted at 1:100.
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