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Application：ELISA
Reactivity：Rat
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：0.9 pg/ml
Storage：2-8℃，6 months
Gene ID：25407
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| Name | Rat CD59 ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | CD59 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Rat |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 1.95-125 pg/ml |
| Sensitivity | 0.9 pg/ml |
| Specificity | Do not react with Rat CD59 analogue etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h |
| Swiss Prot | P27274 |
| Gene ID | 25407 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗大鼠CD59单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的大鼠CD59会与酶标板上的包被抗体充分结合；洗板后加入HRP酶标记的抗大鼠CD59抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的大鼠CD59发生特异性结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的大鼠CD59，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的大鼠CD59浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中大鼠CD59的浓度。 |
| Background | CD59 glycoprotein，also known as 20 kDa homologous restriction factor，HRF20，MAC-inhibitory protein，Membrane attack complex inhibition factor，Membrane inhibitor of reactive lysis，MIC11，MIRL and CD59，is a cell membrane protein which contains one UPAR/Ly6 domain. CD59 is a small，highly glycosylated，GPI-linked protein，with a wide expression profile. The soluble form of CD59 from urine retains its specific complement binding activity，but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. CD59 is a potent inhibitor of the complement membrane attack complex (MAC) action.CD59 was first identified as a regulator of the terminal pathway of complement. It acts by binding to the C8 and/or C9 complements of the assembling MAC，thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be speciesspecific. CD59 is involved in signal transduction for T-cell activation complexed to a protein tyrosinekinase. Defects in CD59 are the cause of CD59 deficiency (CD59D). |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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