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Application：ELISA
Reactivity：Human
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：15.625 pg/ml
Storage：2-8℃，6 months
Gene ID：27074
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| Name | Human LAMP3/CD208 ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | LAMP3/CD208 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Human |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 31.25-2000 pg/ml |
| Sensitivity | 15.625 pg/ml |
| Specificity | Do not react with Human LAMP3 analogue. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h |
| Swiss Prot | Q9UQV4 |
| Gene ID | 27074 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人LAMP3单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的人LAMP3会与酶标板上的包被抗体充分结合；洗板后加入HRP酶标记的抗人LAMP3抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的人LAMP3发生特异性结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的人LAMP3，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的人LAMP3浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中人LAMP3的浓度。 |
| Background | Dendritic cell-lysosomal associated membrane protein (DC-LAMP)/CD208，also known as LAMP3，is a member of the lysosomal associated membrane protein (LAMP) family，which is specifically expressed by human dendritic cells (DCs) upon activation and therefore serves as a marker of human DC maturation. Confocal and immunoelectron microscopy showed that mouse DC-LAMP protein co-localizes with lbm180，a specific marker for the limiting membrane of lamellar bodies that contain surfactant protein B. The present study demonstrates that DC-LAMP is constitutively expressed by mouse，sheep，and human type II pneumocytes. DC-LAMP is constitutively expressed in normal type II pneumocytes. DC-LAMP is detected first in the activated human DC within MHC class II molecules-containing compartments just before the translocation of MHC class II-peptide complexes to the cell surface，suggesting a possible involvement in this process. Furthermore，overexpression of LAMP3 is actively involved in tumor invasion through increased migration into lymph-vascular spaces. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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