{{cart_num}}
My Cart
Application：ELISA
Reactivity：Human
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：31.25 pg/ml
Storage：2-8℃，6 months
Gene ID：2213
Qty：
Size：
| Name | Human Fcgr2b ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | Fcgr2b |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Human |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 62.5-4000 pg/ml |
| Sensitivity | 31.25 pg/ml |
| Specificity | Do not react with Human CD32B analogue etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h |
| Swiss Prot | P31994 |
| Gene ID | 2213 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人CD32B单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的人CD32B会与酶标板上的包被抗体充分结合；洗板后加入HRP酶标记的抗人CD32B抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的人CD32B发生特异性结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的人CD32B，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的人CD32B浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中人CD32B的浓度。 |
| Background | Receptors that recognize the Fc portion of IgG (Fccγ Rs) functions in the regulation of immune response and are divided into three classes designated Fccγ RI (CD64)，Fccγ RII (CD32)，and Fccγ RIII (CD16). Fccγ RI binds IgG with high affinity and functions during early immune responses，whereas Fccγ RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. Three closely related genes encodes human CD32 class by and designated Fccγ RII A，B，and C which share 94 - 99% amino acid identity in their extracellular domains. Fccγ RII B (CD32b) is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. As the only inhibitory member in human，Fccγ RII B has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) and delivers an inhibitory signal upon ligand binding. The coordinated functioning of activating and inhibitory receptors is necessary for successful and proper initiation，amplification，and termination of immune responses，and accordingly，the Fccγ RII B plays an important negativeregulating role through modulating the signals from activation receptors. Recent study has identified CD32b expression in a variety of B-cell malignacies，suggesting that it is a potential immunotherapeutic target for B-cell lymphoproliferative disorders. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Sorry, there is no experimental images.
Sorry, there is no more information.
Sorry, there is no more information.
