W3110(DE3)
Cat.No：C3710 Solarbio
Storage：Store at -70℃
Appearance：Dry ice transport, only 10kg dry ice fee
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Allophycocyanin(APC)-Conjugated
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PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
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MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
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Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
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> Molecular Biology > Recombinant Clone Expression > Competent Cell > W3110(DE3)
Product Information
| Name | W3110(DE3) |
| Appearance | Dry ice transport, only 10kg dry ice fee |
| Storage | Store at -70℃ |
| Prokaryotic Resistance | Sensitive to Amp，Kan，Spe，Str，Gen and Tet |
| Competence | F-λ-rph- 1INV(rrnD，rrnE)( lavUV5::T7 polymerase)(CamR) |
| Product Description | W3110(DE3) was derived from strain K-12, and the bacterial chromosome DNA integrated the original expression frame of T7 RNA polymerase (T7RNP), which was located under the promoter of lacUV5 and could express T7 RNA polymerase. Prokaryotic expression vectors such as pET, pGEX and pMAL can be used to express exogenous recombinant proteins. W3110(DE3) receptive cells were prepared by a special process, and the conversion efficiency of pUC19 plasmid was greater than 1×106CFU/ μg DNA. |
| Note | 1，the receptive cells should be stored at -70℃，not repeatedly frozen and thawed and placed for too long，in order to avoid reducing the conversion efficiency of the receptive cells. 2，the conversion operation should be carried out according to the requirements of the corresponding temperature and aseptic conditions. 3. In order to prevent the conversion experiment from being successful，part of the connected reaction liquid can be retained to re-convert and minimize the loss. |
| Unit | Bag |
| Specification | 20*100ul |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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