Normal Goat Serum
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Cat.No：SL038 Solarbio
Storage：Store at -20℃，5 years
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Secondary Antibodies
SAlexa Fluor-Conjugated
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Other-Conjugated
Colloidal Gold Reagent
Alkaline Phosphatase(AP)-Conjugated
Colloidal Gold-Conjugated
FITC-Conjugated
Biotin-Conjugated
Purified Secondary Antibodies
Allophycocyanin(APC)-Conjugated
CY Fluorescent-Conjugated
Phycoglobin(PE)-Conjugated
RBTIC-Conjugated
Peroxidase (HRP)-Conjugated
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Endocrinology & Hormones
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Cytoskeleton
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Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
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Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
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> Protein and Immunity > Other Reagents > Other Buffers/Reagents > Normal Goat Serum
Product Information
| Name | Normal Goat Serum |
| Storage | Store at -20℃，5 years |
| Unit | Bottle |
| Specification | 10ml 100ml |
The sealed serum is suitable for sealing samples in immunohistochemical or immunofluorescence tests. It can block the non-specific binding sites of proteins in the samples to be tested, and can also block the binding sites of endogenous Fc fragments in the samples to block the binding of antibodies to Fc receptors in the samples, thereby reducing the background and reducing false positives. When selecting the blocking serum, it should be noted that the blocking serum cannot contain the target protein, and the source is different from the primary antibody, and the blocking serum from the same source as the second antibody can be used.
Usage:
When used, the sealed serum stock solution is diluted 10 or 20 times with PBS (0.01mol/LpH7.2-7.4) to form 10% or 5% working solution, which is directly added to the tissue sections or cell sheets, incubated at 37℃ for 10-30 minutes, the serum is removed, do not wash, and the appropriate proportion of diluted primary antibody working solution is added. Incubation at 37℃ for 1-2h or overnight at 4℃.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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