Lycopene

Examples of using this product（for reference only）

UPLC

Chromatography was carried out with a Ultra-high performance liquid chromatography. Samples were measured by a C18 carotenoid column (100 mm × 2.1 i.d., 1.7 μm). The eluent was methyl tertiary butyl ether and methanol (V/V = 30:70) with the type of isocratic elution. And each eluent contained 0.01% BHT and 0.05% TEA (triethylamine) as modifiers in order to prevent the degradation of carotenoids on the column. The following rate was 0.5 mL/min, column temperature was 25 ℃, and the injection volume was 5 μL, analyzing with UV at 445 nm.

References：

Shen YH, Yang FY, Lu BG, Zhao WW, Jiang T, Feng L, Chen XJ, Ming R. Exploring the differential mechanisms of carotenoid biosynthesis in the yellow peel and red flesh of papaya. BMC Genomics. 2019 Jan 16;20(1):49. doi: 10.1186/s12864-018-5388-0. PMID: 30651061; PMCID: PMC6335806.

LC-MS

The carotenoids contents were determined using an HPLC system equipped with a C30 column (150 ×4.6 mm, 3 μm) and a UV/VIS detector. The mobile phase consisted ofA (acetonitrile: methanol = 3:1) and B (methyl tert-butyl ether), and a gradient elution program (Lee et al., 2001) was used. The flow rate was 1.0 ml/min, and the column temperature was 40℃. The signals were detected at 450 nm. Identification of lutein and zeaxanthin was conducted on AB TripleTOF 5600 plus system equipped with an APCI source operated in the positive ionization mode. The column and temperature settings were the same as HPLC. The mobile phase was A (methanol) and B (acetonitrile: isopropanol = 1:1) with linear increase of B from 0 to 100% in 30 min followed by dropping to 0 in 10 min. The source voltage was +5.5 kV, and the source temperature was 500℃. The pressure of Gas 1 (Air) and Gas 2 (Air) was set to 50 psi. The pressure of Curtain Gas (N2) was set to 35 psi. The m/z scan range of production was set as 50–1500.

References：

Bian Q, Zhou P, Yao Z, Li M, Yu H, Ye L. Heterologous biosynthesis of lutein in S. cerevisiae enabled by temporospatial pathway control. Metab Eng. 2021 Sep;67:19-28. doi: 10.1016/j.ymben.2021.05.008. Epub 2021 May 30. PMID: 34077803.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.