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My CartReactivity：Human,Rat,Mouse
Application：IHC,IF,IP,FCM,WB
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：5970
Host：Rabbit
Qty：
Size：
| Name | Anti-NF-kB p65 Monoclonal Antibody |
| Host | Rabbit |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:1000-5000. IHC 1:50-200. IF 1:50-200. FCM 1:1000-3000. |
| Target | NF-kB p65 |
| Background | Proteins encoded by the v-Rel viral oncogene and its cellular homolog，c-Rel，are members of a family of transcription factors that include the two subunits of the transcription factor NF kB (p50 and p65) and the Drosophila maternal morphogen，dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp kB sequence in the immunoglobulin k light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NFkB is activated and NFkB is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described，designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule，designated pdI，binds to p50 and regulates its activity. |
| Swiss Prot | Q04206 |
| Gene ID | 5970 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | A synthetic peptide of human NF-kB p65 |
| Public Immunogen Range | 490-540aa |
| Calculated MW | 65kDa |
| Purification Method | Affinity purification |
| Storage Buffer | 1*TBS (pH7.4)，0.05% BSA，40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human Rat Mouse |
| Application | WB FCM IP IF IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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