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My CartReactivity：Rat,Human,Mouse
Application：IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：9040
Host：Rabbit
Qty：
Size：
| Name | Anti-UBE2M Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Bovine |
| Dilution Ratio | WB 1:1000-3000.IHC 1:50-200. |
| Target | UBE2M |
| Background | Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1，but not RBX2，suggests that the RBX1-UBE2M complex neddylates specific target proteins，such as CUL1，CUL2，CUL3 and CUL4. Involved in cell proliferation. |
| Swiss Prot | P61081 |
| Gene ID | 9040 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | Recombinant protein of human UBE2M |
| Public Immunogen Range | 1-184aa |
| Calculated MW | 21kDa |
| Observed MW | 19kDa |
| Purification Method | Octanoic acid-ammonium sulfate precipitation |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Rat Mouse |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with UBE2M antibody diluted at 1:2000;Lane: 293T,HepG2,PC12,MG-63,HeLa,SW480,HT-29.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with UBE2M antibody diluted at 1:100.
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