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My CartReactivity：Rat,Human,Mouse
Application：IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：89
Host：Rabbit
Qty：
Size：
| Name | Anti-ACTN3 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:1000-3000.IHC 1:50-200. |
| Target | ACTN3 |
| Background | This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status. [provided by RefSeq，Feb 2014] |
| Swiss Prot | Q08043 |
| Gene ID | 89 |
| Subcellular Location | Cytoplasm |
| Immunogen | Recombinant protein of human ACTN3 |
| Public Immunogen Range | 301-650aaa |
| Calculated MW | 103kDa |
| Observed MW | 100kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Rat Mouse |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with ACTN3 antibody diluted at 1:2000;Lane: COLO205,L-02,HT29,HeLa,HepG2,PC-12,Mouse brain,Mouse lung,Rat spleen,Rat skeletal muscle.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with ACTN3 antibody diluted at 1:100.
Immunohistochemistry of paraffin-embedded Human thyroid cancer with ACTN3 antibody diluted at 1:100.
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