| Chinese Name |
五因子检测试剂盒 |
| English Name |
Human Cytokine FlowCytometric Bead Array(CBA) Kit |
| Optional Factor Category |
TNF-α/IFN-γ/IL-1β/IL-2/IL-4/IL-6/IL-8/IL-10/IL-12p70/IL-17 |
| Detection Sample |
Human Serum, Plasma, Culture Supernatant |
| Adaptive Model |
This product is suitable for most flow cytometers with PE and APC channels on the market. |
| Sample Requirement |
Serum, plasma, cell culture supernatant: It is recommended to test within 4-6 hours, if it cannot be tested in time, please freeze at -24℃, avoid repeated freezing and thawing. |
| Detection Method |
See the instructions for details |
| Reference Interval |
See the instructions for details |
| Unit |
Kit |
| Optional Factor Number |
Five Factors |
| Sample Species |
Human |
| Detection Principle |
This kit uses CBA multifactor flow detection technology, and the fluorescence codes of microspheres are different. Package is respectively on different microspheres with TNF alpha/IFN - gamma/IL - 1 beta/IL - 2 / IL - 4 / IL - 6, IL - 8 / IL - 10 per IL - 12 p70 / IL - 17 specific antibodies, and incubated with the sample under test, with the sample of TNF alpha/IFN - gamma/IL - 1 beta/IL - 2 / IL - 4 / IL - 6 /IL-8/IL-10/IL-12p70/IL-17 specific binding, followed by the addition of biotin-labeled detection antibody, antibody coated microsphere-cytokine - detection antibody immune complex; Finally, phycoregiin-labeled streptavidin was added and combined with biotin. The fluorescence intensity of the tested substance was obtained by flow cytometry. The fluorescence intensity was proportional to the content of TNF-α/IFN-γ/IL-1β/IL-2/IL-4/IL-6/IL-8/IL-10/IL-12p70/IL-17 in the sample. Finally, the standard curves of these ten cytokine standards were combined to achieve the quantitative detection of TNF-α /IFN-γ/IL-1β/IL-2/IL-4/IL-6/IL-8/IL-10/IL-12p70/IL-17 in the same sample. So as to help judge the immune function state of the body. |
| Component 1 |
Mixture of Trapping Microsphere |
| Component 2 |
Mixture of Detect Antibody |
| Component 3 |
Standard |
| Component 4 |
PE-labeled Streptavidin |
| Component 5 |
Blank Microsphere |
| Component 6 |
Standard/Sample Diluent |
| Component 7 |
20× Washing Solution |
| Component 8 |
Microporous Plate |
| Component 9 |
Sealing Film |
| Specification |
48T 96T |
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