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My CartReactivity：Rat,Mouse,Human
Application：IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：5580
Host：Rabbit
Qty：
Size：
| Name | Anti-PRKCD Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Dog |
| Dilution Ratio | WB 1:1000-3000.IHC 1:25-100. |
| Target | PRKCD |
| Background | Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008] |
| Swiss Prot | Q05655 |
| Gene ID | 5580 |
| Subcellular Location | Cytoplasm Cell membrane Nucleus |
| Immunogen | Recombinant protein of human PRKCD |
| Public Immunogen Range | 30-380aa |
| Calculated MW | 78/81kDa |
| Observed MW | 75kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.03% Proclin300, 0.5% BSA, pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Mouse Rat |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with PRKCD antibody diluted at 1:2000;Lane: SH-SY5Y,THP-1.
Immunohistochemistry of paraffin-embedded Human ovarian cancer with PRKCD antibody diluted at 1:50.
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