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My CartReactivity：Mouse,Human
Application：IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：10865
Host：Rabbit
Qty：
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| Name | Anti-ARID5A Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:1000-3000.IHC 1:50-200. |
| Target | ARID5A |
| Background | Binds to AT-rich stretches in the modulator region upstream of the human cytomegalovirus major intermediate early gene enhancer. May act as repressor and down-regulate enhancer-dependent gene expressison (PubMed:8649988).May positively regulate chondrocyte-specific transcription such as of COL2A1 in collaboration with SOX9 and positively regulate histone H3 acetylation at chondrocyte-specific genes. May stimulate early-stage chondrocyte differentiation and inhibit later stage differention (By similarity).Can repress ESR1-mediated transcriptional activation;proposed to act as corepressor for selective nuclear hormone receptors (PubMed:15941852).As RNA-binding protein involved in the regulation of inflammatory response by stabilizing selective inflammation-related mRNAs, such as IL6, STAT3 and TBX21. Binds to stem loop structures located in the 3'UTRs of IL6, STAT3 and TBX21 mRNAs;at least for STAT3 prevents binding of ZC3H12A to the mRNA stem loop structure thus inhibiting its degradation activity. Contributes to elevated IL6 levels possibly implicated in autoimmunity processes. IL6-dependent stabilization of STAT3 mRNA may promote differentiation of naive CD4+ T-cells into T-helper Th17 cells. In CD4+ T-cells may also inhibit RORC-induced Th17 cell differentiation independently of IL6 signaling. Stabilization of TBX21 mRNA contributes to elevated interferon-gamma secretion in Th1 cells possibly implicated in the establishment of septic shock (By similarity).Stabilizes TNFRSF4/OX40 mRNA by binding to the conserved stem loop structure in its 3'UTR;thereby competing with the mRNA-destabilizing functions of RC3H1 and endoribonuclease ZC3H12A (By similarity). |
| Swiss Prot | Q03989 |
| Gene ID | 10865 |
| Subcellular Location | Nucleus |
| Immunogen | Recombinant protein of human ARID5A |
| Public Immunogen Range | 1-347aa |
| Calculated MW | 57/64kDa |
| Observed MW | 62kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.03% Proclin300, 0.5% BSA, pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Mouse |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Immunohistochemistry of paraffin-embedded Human renal cancer with ARID5A antibody diluted at 1:100.
Western blot analysis with ARID5A antibody diluted at 1:2000;Lane: A431.
Immunohistochemistry of paraffin-embedded Human liver cancer with ARID5A antibody diluted at 1:100.
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