{{cart_num}}
My CartReactivity：Rat,Human,Mouse
Application：IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：1727
Host：Rabbit
Qty：
Size：
| Name | Anti-CYB5R3 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Monkey;Pig |
| Dilution Ratio | WB 1:1000-3000.IHC 1:50-200. |
| Target | CYB5R3 |
| Background | This gene encodes cytochrome b5 reductase，which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum，mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids，in cholesterol biosynthesis，and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains，while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in this gene cause methemoglobinemias. |
| Swiss Prot | P00387 |
| Gene ID | 1727 |
| Subcellular Location | Cytoplasm |
| Immunogen | A synthetic peptide of human CYB5R3 |
| Public Immunogen Range | 220-270/301aa |
| Calculated MW | 32/34/38kDa |
| Observed MW | 34kDa |
| Purification Method | Octanoic acid-ammonium sulfate precipitation |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | bottle |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Rat Mouse |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with CYB5R3 antibody diluted at 1:2000;Lane: HepG2,HeLa,U87,MG63,A498,THP-1,Raji,Lung cancer.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with CYB5R3 antibody diluted at 1:100.
Sorry, there is no more information.
Sorry, there is no more information.
Manual Download
