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My CartReactivity：Human
Application：WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：23532
Host：Rabbit
Qty：
Size：
| Name | Anti-PRAME Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:1000-3000. |
| Target | PRAME |
| Background | This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues，except testis. The encoded protein acts as a repressor of retinoic acid receptor，and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants. |
| Swiss Prot | P78395 |
| Gene ID | 23532 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | A synthetic peptide of human PRAME |
| Public Immunogen Range | 459-509/509aa |
| Calculated MW | 58kDa |
| Observed MW | 61kDa |
| Purification Method | Octanoic acid-ammonium sulfate precipitation |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | bottle |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human |
| Application | WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with PRAME antibody diluted at 1:2000;Lane: U-251MG,MG-63.
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