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My CartReactivity：Mouse,Rat,Human
Application：IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：284654
Host：Rabbit
Qty：
Size：
| Name | Anti-RSPO1 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:1000-3000.IHC 1:50-200. |
| Target | RSPO1 |
| Background | This gene encodes a secreted activator protein with two cysteine-rich，furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice，the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing，providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants. |
| Swiss Prot | Q2MKA7 |
| Gene ID | 284654 |
| Subcellular Location | Secreted |
| Immunogen | A synthetic peptide of human RSPO1 |
| Public Immunogen Range | 20-70/263aa |
| Calculated MW | 22/26/29kDa |
| Observed MW | 20/29kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | bottle |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Mouse Rat |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with RSPO1 antibody diluted at 1:2000;Lane: NIH3T3,SKOV3,THP-1.
Immunohistochemistry of paraffin-embedded Human breast cancer with RSPO1 antibody diluted at 1:100.
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