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My CartReactivity：Human
Application：IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：3848
Host：Rabbit
Qty：
Size：
| Name | Anti-KRT1 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:1000-3000.IHC 1:50-200. |
| Target | KRT1 |
| Background | The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. |
| Swiss Prot | P04264 |
| Gene ID | 3848 |
| Subcellular Location | Cytoplasm Nucleus Secreted |
| Immunogen | A synthetic peptide of human KRT1 |
| Public Immunogen Range | 200-250/644aa |
| Calculated MW | 66kDa |
| Observed MW | 70kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | bottle |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with KRT1 antibody diluted at 1:2000;Lane: MCF7,HEK293.
Immunohistochemistry of paraffin-embedded Human stomach cancer with KRT1 antibody diluted at 1:100.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with KRT1 antibody diluted at 1:100.
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