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My CartReactivity：Mouse,Monkey,Human,Rat
Application：IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：3091
Host：Rabbit
Qty：
Size：
| Name | Anti-HIF1A Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Cow |
| Dilution Ratio | WB 1:2000-5000.IHC 1:25-100. |
| Target | HIF1A |
| Background | This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1)，which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes，including those involved in energy metabolism，angiogenesis，apoptosis，and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization，tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. |
| Swiss Prot | Q16665 |
| Gene ID | 3091 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | A synthetic peptide of human HIF1A |
| Public Immunogen Range | 200-250/826aa |
| Calculated MW | 83/93/97kDa |
| Observed MW | 120kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | bottle |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Monkey Rat Mouse |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with HIF1A antibody diluted at 1:3000;Lane: COS7,Jurkat,MCF7,HCT116,A431,U251,U87,COS7.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with HIF1A antibody diluted at 1:50.
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