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My CartReactivity：Mouse,Rat,Human
Application：IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：9798
Host：Rabbit
Qty：
Size：
| Name | Anti-IST1 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Bovine;Gorilla |
| Dilution Ratio | WB 1:1000-3000.IHC 1:50-200. |
| Target | IST1 |
| Background | This gene encodes a protein with MIT-interacting motifs that interacts with components of endosomal sorting complexes required for transport (ESCRT). ESCRT functions in vesicle budding，such as that which occurs during membrane abscission in cytokinesis. There is a pseudogene for this gene on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| Swiss Prot | P53990 |
| Gene ID | 9798 |
| Subcellular Location | Nucleus Cytoplasm |
| Immunogen | Recombinant protein of human IST1 |
| Public Immunogen Range | 1-364aa |
| Calculated MW | 23/37/40/42kDa |
| Observed MW | 46kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | bottle |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Mouse Rat |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with IST1 antibody diluted at 1:2000;Lane: A549,HeLa,PC-3,NIH3T3,HEK293,Jurkat,Mouse brain,Mouse lung.
Immunohistochemistry of paraffin-embedded Human stomach cancer with IST1 antibody diluted at 1:100.
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