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My CartReactivity：Mouse,Rat,Human
Application：IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：5352
Host：Rabbit
Qty：
Size：
| Name | Anti-PLOD2 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:1000-3000.IHC 1:50-200. |
| Target | PLOD2 |
| Background | The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. Mutations in the coding region of this gene are associated with Bruck syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Swiss Prot | O00469 |
| Gene ID | 5352 |
| Subcellular Location | Cytoplasm |
| Immunogen | Recombinant protein of human PLOD2 |
| Public Immunogen Range | 1-350aa |
| Calculated MW | 49/85/87kDa |
| Observed MW | 95kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | bottle |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Mouse Rat |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with PLOD2 antibody diluted at 1:2000;Lane: A549,Hela.
Immunohistochemistry of paraffin-embedded Human ovarian cancer with PLOD2 antibody diluted at 1:100.
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