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My Cart> Cell Biology > Cell Detection > Cell Growth Factor > Recombinant Human Actin, cytoplasmic 1/ACTBPurity：≥95% as determined by reducing SDS-PAGE.
Storage：Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Appearance：Supplied as a 0.2 μm filtered solution of 10mM TrisHCl, 0.1% TritonX-100, 2mM DTT, pH 8.0.
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| Appearance | Supplied as a 0.2 μm filtered solution of 10mM TrisHCl, 0.1% TritonX-100, 2mM DTT, pH 8.0. |
| Purity | ≥95% as determined by reducing SDS-PAGE. |
| Storage | Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| Transport Condition | The product is shipped on dry ice pack. |
| Background | Actins are ubiquitous globular and highly conserved proteins that are involved in various types of cell motility，structure，and integrity.Three main groups of actin isoforms，alpha，beta and gamma have been identified.The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus.The beta and gamma actins co-exist in most cell types as components of the cytoskeleton，and as mediators of internal cell motility.ACTB is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix.Each actin can bind to 4 others. |
| Source | E.coli |
| Endotoxin | Less than 0.1 ng/ug(1 IEU/ug)as determined by LAL test. |
| Unit | Piece |
| Specification | 10ug 50ug 500ug 1mg |
Recombinant Human beta-Actin is produced by our E.coli expression system and the target gene encoding Asp2-Phe375 is expressed with a 6His tag at the C-terminus.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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