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My CartReactivity：Mouse,Rat,Human
Application：IHC
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：6469
Purity：SHH
Qty：
Size：
| Name | Anti-SHH Polyclonal Antibody |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | IHC 1:50-200. |
| Target | Rabbit |
| Background | This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube， the anterior-posterior limb axis， and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila， this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly， the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product， restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE)， a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome， which is characterized by vertebral defects， anal atresia， tracheoesophageal fistula with esophageal atresia， radial and renal dysplasia， cardiac anomalies， and limb abnormalities. Additionally， mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly. |
| Swiss Prot | Q15465 |
| Gene ID | 6469 |
| Subcellular Location | Cytoplasm Cell membrane Secreted |
| Immunogen | A synthetic peptide of human SHH |
| Public Immunogen Range | 50-100/462aa |
| Calculated MW | 50kDa |
| Observed MW | Chicken;Newt;Xenopus;laevis |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol， 0.03% Proclin300， 0.5% BSA， pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Purity | SHH |
| Conjugation Concentration | bottle |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Mouse Rat |
| Application | IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
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