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My Cart> Biochemical Assay Kits > REDOX Series > Glutathione Series > Glutathione peroxidase 4（GPX4）Activity Assay Kit
Detection Method：Spectrophotometer/Microplate Reader
Storage：Store at -20℃，6months
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| Reagents Required But Not Provided | If you use 96-well plate determination, you need to use 96-well UV plate, recommended cat. YA0602 |
| Name | Glutathione peroxidase 4（GPX4）Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6months |
| Unit | kit |
| Specification | 100T/48S |
Glutathione peroxidase (GPX, EC 1.11.1.9) is an important peroxidase-degrading enzyme that widely exists in the body. It can catalyze the reaction of glutathione (GSH) with peroxides and reduce peroxides to hydroxyl compounds, thereby protecting cells from free radical damage.
Glutathione peroxidase catalyzes the reaction of GSH with peroxide to form oxidized glutathione (GSSG). GSSG reacts with NADPH to form GSH under the action of glutathione reductase (GR). NADPH has a characteristic absorption peak at 340 nm. The rate of absorbance decrease in the reaction system is linearly related to the activity of glutathione peroxidase. The kit calculates the activity of GPX by measuring the consumption of NADPH per unit time.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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