SYBR Green I(10000×)

Specification: 50/100μL

Storage: Store at -20℃ away from light, valid for 12 months.

Product Introduction:

When using agarose electrophoresis to detect double-stranded DNA, SYBR GreenⅠ is the most sensitive fluorescent dye. When it binds to nucleic acid, it will produce strong fluorescence and high quantum yield. The quantum yield of DNA/SYBR GreenⅠ complex is 0.8. Due to the strong signal produced by binding to nucleic acids, extremely low background, and high affinity for nucleic acids, SYBR dyes can be used in low concentration conditions. The detection limit of SYBR GreenⅠ was 20pg DNA (254nm). 60pg DNA (300nm ultraviolet transmission); In addition, SYBR GreenⅠ can also be used to detect oligonucleotides (1-2ng, 300nm UV transmission), which is 20 to 100 times more sensitive than EB. SYBR GreenⅠ is used for DNA detection by electrophoresis, which can be either pre-dyed or dyed after electrophoresis. After SYBR GreenⅠ is used for DNA detection by agalose electrophoresis, the DNA can be directly transferred to the membrane for subsequent nucleic acid blot hybridization. In addition, the binding of SYBR GreenⅠ to DNA has no inhibition on the activity of many commonly used restriction enzymes, and can be directly digested or linked.

Instructions for use:

The product is used in the same way as EB. Staining method: For agar-gel electrophoresis: Take 1μl of raw solution and add TE buffer or sterilized double steam 1mL, then add 1mL of 6×DNA Loading buffer and mix well. (The solution is 1: 2000 dilution, that is, the working liquid) During electrophoresis, take 1-2μL of the working liquid and 5μL of the electrophoresis sample, mix it well, stand for 5 minutes, and then directly sample. Gel dyeing method: Conventional configuration of agar-agar gel 100mL, heating to melt, when the temperature is 50-70℃ (does not feel hot) add the stock solution 10μL. After the gel solidifies, it can be electrophoresis, and after electrophoresis, it can be observed under ultraviolet irradiation.

Note:

1. SYBR GreenⅠ should be stored away from light, and the stock solution should be stored at -20℃; It is recommended to pack and freeze, which can be stored at 4℃ for a short time.

2. The glue dyeing method is highly sensitive, and the commercial DNA marker must be diluted 5-10 times before use.

3. In the pre-staining method, the electrophoresis time should not exceed 2h, otherwise SYBR GreenⅠ will be separated from the DNA, resulting in a diffuse band.

4. In the process of conventional precipitation of nucleic acid with alcohol, SYBR Green I can be completely removed from double-stranded nucleic acid.

5. Under ultraviolet irradiation, SYBR Green I conjugated with double-stranded DNA showed green fluorescence. If the glue contains single chains; The DNA is orange instead of green.

6. SYBR Green has a certain affinity for glass and non-polypropylene materials. It is recommended to use polypropylene containers during dilution, storage, dyeing, etc.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.