Dnase Agar Base with Methyl Green
Cat.No：LA0850 Solarbio
Storage：RT，keep dry
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> Microbial Culture > Other Microbial Medium > Dnase Agar Base with Methyl Green
Product Information
| Name | Dnase Agar Base with Methyl Green |
| Storage | RT，keep dry |
| Grade | BR |
| Unit | Bottle |
| Specification | 100g |
Product introduction
dnase for Campylobacter
preparation method
42.0g of this product was weighed and dissolved in 1000ml distilled water by slow heating. The product was divided into triangular bottles and autoclaved at 121℃ for 15 minutes, and cooled to about 50℃. For every 100ml of medium, 1ml of 0.5% methyl green water solution filtered to remove bacteria was added, mixed, and poured into the plate.
composition: g/L
DNA: 2.0
Phytopeptone: 5.0
Pancreatic casein peptone: 15.0
Sodium chloride: 5.0
Agar: 15.0
pH: 7.3±0.1, 25℃
Precautions
Methyl green preparation (MG) : 0.5% methyl green aqueous solution was prepared. The same volume of chloroform was extracted 5-7 times until the chloroform layer was colorless. 1ml of staining solution was added to each 100ml of sterilized dissolved medium, and the final concentration of methyl green was 0.005%. Methyl green should be prepared by yourself.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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