{{cart_num}}
My CartReactivity：Mouse
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：15.63 pg/ml
Storage：2-8℃，6 months
Gene ID：16160
Qty：
Size：
| Name | Mouse IL-12/IL-23 p40 ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | IL-12 p40 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Mouse |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 31.25-2000 pg/ml |
| Sensitivity | 15.63 pg/ml |
| Specificity | Do not react with Human IL-12/IL-23 p40、IL-12 p70、IL-23 etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h |
| Swiss Prot | P43432 |
| Gene ID | 16160 |
| Detection Principle | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-12/IL-23 p40 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells; any IL-12/IL-23 p40 present is captured by the coated antibody after incubation. After washing away any unbound substances, a biotin-conjugate antibody specific for IL-12/IL-23 p40 is added to detect the captured IL-12/IL-23 p40 protein in the sample. Following a wash to remove any unbound combination, horseradish peroxidase (HRP)-conjugated Streptavidin is added to the wells. After extensive washing, a tetramethyl-benzidine (TMB) reagent is added to the wells for signal development. Solution containing sulfuric acid is used to stop color development. The color intensity, proportional to the quantity of bound protein, is then measurable at 450nm. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Sorry, there is no experimental images.
Sorry, there is no more information.
Characterization of genetic humanized mice with transgenic HLA DP401 or DRA but deficient in endogenous murine MHC class II genes upon Staphylococcus aureus pneumonia
Click to check >>Author：Feng Li, Bowen Niu, Lingling Liu, Mengmin Zhu, Hua Yang, Boyin Qin, Xiuhua Peng, Lixiang Chen, Chunhua Xu, Xiaohui Zhou
IF：0.0000
Publish_to：Animal Models and Experimental Medicine
PMID：37246733
